Incorporation of DQ™ collagen type I bovine skin, fluorescein conjugate into RASTRUM™ Matrix

This protocol outlines the incorporation of DQ™ Collagen Type I Bovine Skin, Fluorescein Conjugate into RASTRUM™ Matrix.

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Overview

DQ™ Collagen Type I Fluorescein Conjugate can be used to directly monitor collagenase activity of the cells encapsulated in RASTRUM™ advanced cell models. The generation of fluorescein signals is driven by the enzyme-driven hydrolysis of the substrate that separates the dye molecules from one another. Herein, we have developed a simple protocol to incorporate DQ™ Collagen Type I Fluorescein Conjugate into RASTRUM™ Matrix.

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