Assessing the viability of cells within RASTRUM™ 3D cell models is a fundamental practice for determining the health of 3D cell cultures. Additionally, viability assessments serve as the foundation for toxicology and efficacy studies of malignant cells.
In this protocol, we describe in situ staining of live advanced cell high-throughput models created with RASTRUM™ (HepG2 spheroids) treated with 50 mM acetaminophen using PhenoVue™ Live/Dead Cell Viability Assay Kit (Revvity). While this protocol references the HTP model, it can be easily adapted for other RASTRUM™ 3D Advanced Cell Models.