Small and large molecule drug discovery projects, particularly high-throughput screening, widely use high-sensitivity protein assays such as Alpha (amplified luminescence proximity homogeneous assay). Alpha is a bead-based assay platform employing oxygen-channelling chemistry for the detection and quantification of various biomolecules. Herein, we describe a protocol for the Alpha assay using RASTRUM 3D cell models.
AlphaLISA® SureFire® Ultra™ Assay for RASTRUM™ 3D Cell Models
A protocol for the Alpha assay using RASTRUM 3D cell models.