Overview

Cryosections offer a rapid and relatively straightforward sample preparation, making them ideal for visualising intricate cellular details. While cryosections may be physically less stable compared to sections embedded in paraffin or resin, they generally excel in preserving antigenicity, crucial for antigen detection through microscopy. Cryosection preparation doesn't involve the dehydration steps found in other sectioning methods, and sectioning, labelling, and specimen observation can be completed in a single day. Frozen sections are versatile and can be used for various procedures, including immunochemistry, enzymatic detection, and in situ hybridisation. Here we provide a protocol to cryosection RASTRUM™ 3D cell models to easily adopt this technique.

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