protocols

Our in-house curated application notes highlight the applicability and capability of the RASTRUM™ Platform.
Discover how the platform can be utilised for a particular application to support impactful scientific research.

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Handling RASTRUM™ Removable Models

A protocol for the process of handling the RASTRUM™ Removable Models to allow for the implementation of these techniques, such as immunohistochemistry, light-sheet microscopy, advanced tissue-omics and transplantation.
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PhenoVue™ In Situ Live/Dead Cell Viability with RASTRUM™ 3D Advanced Cell Models

This protocol outlines an easy-to-use method for measuring cell viability, cell proliferation, cytotoxicity and apoptosis using the PhenoVue™ Live/Dead Cell Viability Assay Kit.
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Incorporation of DQ™ collagen type I bovine skin, fluorescein conjugate into RASTRUM™ Matrix

This protocol outlines the incorporation of DQ™ Collagen Type I Bovine Skin, Fluorescein Conjugate into RASTRUM™ Matrix.
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RNA extraction from RASTRUM™ Advanced Cell Models using Promega ReliaPrep™ System

A simple step-by-step protocol to extract high quality and purity RNA from a RASTRUM™ advanced cell models for RT-qPCR or RNA sequencing downstream analysis.
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AlphaLISA® SureFire® Ultra™ Assay for RASTRUM™ 3D Cell Models

A protocol for the Alpha assay using RASTRUM™ 3D cell models.
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In situ CellTiter-Glo® 3D assay for RASTRUM™ Advanced Cell Models

This protocol outlines the use of CellTiter-Glo® 3D assay to assess the cell viability in RASTRUM™ advanced cell models.
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Protein Extraction & Quantification from RASTRUM™ 3D Cell Models

This protocol details how to extract and quantify proteins from RASTRUM™ 3D cell models.
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In situ viability analysis of RASTRUM™ 3D cell models

This protocol outlines a method to assess viability of 3D cell models created with RASTRUM™ using fluorescent dyes for distinguishing live and dead cells via fluorescence microscopy.
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ELISA Assay for RASTRUM™ 3D Cell Models

This protocol outlines a method for enzyme-linked immunosorbent assays (ELISA) to assess the proteins secreted by cells in 3D cell models created with RASTRUM™ to provide data on the response from cells in disease state or drug treatment.
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