Protocols
Our easy-to-follow protocols are written by our scientists for scientists.
Explore how simple it is to integrate RASTRUM™ into your existing experimental workflows with our protocols.
This protocol outlines how the enzymatic-based RASTRUM™ Cell Retrieval Fluid can be used to extract advanced cell models encapsulated in RASTRUM™ Matrices for use in downstream applications.
This protocol outlines a method to assess viability of 3D cell models created with RASTRUM™ using fluorescent dyes for distinguishing live and dead cells via fluorescence microscopy.
This protocol outlines the incorporation of DQ™ Collagen Type I Bovine Skin, Fluorescein Conjugate into RASTRUM™ Matrix.
A simple step-by-step protocol to extract high quality and purity RNA from a RASTRUM™ advanced cell models for RT-qPCR or RNA sequencing downstream analysis.
This protocol outlines how qPCR gene expression analysis can be used with RASTRUM™ advanced cell models.
This protocol outlines an easy-to-use method for measuring cell viability, cell proliferation, cytotoxicity and apoptosis using the PhenoVue™ Live/Dead Cell Viability Assay Kit.
This protocol outlines a method for enzyme-linked immunosorbent assays (ELISA) to assess the proteins secreted by cells in 3D cell models created with RASTRUM™ to provide data on the response from cells in disease state or drug treatment.
This protocol details how to extract and quantify proteins from RASTRUM™ 3D cell models.
This protocol outlines a method to analyse protein expression via immunofluorescence in 3D cell models created with RASTRUM™