Processing and Paraffin-Embedding of RASTRUM™ Removable Model
This protocol outlines a method to perform immunohistochemistry on formalin-fixed paraffin embedded RASTRUM™ Removable Models.
Handling RASTRUM™ Removable Models
A protocol for the process of handling the RASTRUM™ Removable Models to allow for the implementation of these techniques, such as immunohistochemistry, light-sheet microscopy, advanced tissue-omics and transplantation.
PhenoVue™ In Situ Live/Dead Cell Viability with RASTRUM™ 3D Advanced Cell Models
This protocol outlines an easy-to-use method for measuring cell viability, cell proliferation, cytotoxicity and apoptosis using the PhenoVue™ Live/Dead Cell Viability Assay Kit.
Incorporation of DQ™ collagen type I bovine skin, fluorescein conjugate into RASTRUM™ Matrix
This protocol outlines the incorporation of DQ™ Collagen Type I Bovine Skin, Fluorescein Conjugate into RASTRUM™ Matrix.
RNA extraction from RASTRUM™ Advanced Cell Models using Promega ReliaPrep™ System
A simple step-by-step protocol to extract high quality and purity RNA from a RASTRUM™ advanced cell models for RT-qPCR or RNA sequencing downstream analysis.
AlphaLISA® SureFire® Ultra™ Assay for RASTRUM™ 3D Cell Models
A protocol for the Alpha assay using RASTRUM™ 3D cell models.
In situ CellTiter-Glo® 3D assay for RASTRUM™ Advanced Cell Models
This protocol outlines the use of CellTiter-Glo® 3D assay to assess the cell viability in RASTRUM™ advanced cell models.
Protein Extraction & Quantification from RASTRUM™ 3D Cell Models
This protocol details how to extract and quantify proteins from RASTRUM™ 3D cell models.
In situ viability analysis of RASTRUM™ 3D cell models
This protocol outlines a method to assess viability of 3D cell models created with RASTRUM™ using fluorescent dyes for distinguishing live and dead cells via fluorescence microscopy.
ELISA Assay for RASTRUM™ 3D Cell Models
This protocol outlines a method for enzyme-linked immunosorbent assays (ELISA) to assess the proteins secreted by cells in 3D cell models created with RASTRUM™ to provide data on the response from cells in disease state or drug treatment.