Overview
Need to recover cells from your RASTRUM™ models for downstream analysis? This protocol outlines a rapid, reproducible workflow for retrieving live single cells or intact spheroids from synthetic hydrogel matrices using RASTRUM Cell Retrieval Solution (CRS).
Whether you’re preparing samples for flow cytometry, gene expression, or further culturing, this step-by-step guide supports high-efficiency retrieval across multiple RASTRUM formats all while preserving biological fidelity. Optimized for flexibility, the method enables recovery of both spheroid aggregates and dissociated single cells with minimal disruption.
Includes incubation times, reagent volumes, and best practices for working with different matrix stiffnesses and model architectures.
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