3D Cell Culture in Drug Discovery Explained
The extracellular matrix.....
Stiffness
Key molecules
Other
Plate format and model architecture will define the power of your experiments.
Simple basement membrane extract (BME) domes were introduced in 2009, and have come a long way since then. Now advanced methods like bioprinting can create reproducible, scalable models for high-throughput drug screenings.
These models gain further utility with advanced architectures that are optimized for high throughput imaging or specific assays including histology, migration assays, or invasion assays.
Optimising the model to fit the analysis
For advanced drug discovery, researchers should begin their design of experiments with a question they are trying to solve. From there, a series of analyses is identified which will answer the question. Once the analysis is identified, 3D cell models can be optimised to best fit the analysis.
[reword, but that's the general idea]
Don't know where to start?
- Compare various 3D cell models to learn which is best suited for your research
- Learn about the differences between 2D and 3D cell culture, and how to adapt downstream workflows
Drug discovery pipeline
- Tune matrix stiffness, protein and peptide content in RASTRUM™ Matrices to recapitulate your target cellular phenotype
- Avoid uncontrolled fibroblast activation in RASTRUM™ Matrices, which is commonly observed in basement membrane extract
- No matrix batch-to-batch variability, resulting in meaningful, reproducible and standardised experiments
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Enabling high throughput drug discovery in 3D cell cultures through a novel bioprinting workflow
SLAS Technology | February 2022
High throughput characterization of advanced 3D liver models for in vitro hepatotoxicity studies
Poster
Generation and analysis of 3D cell culture models for drug discovery
European Journal of Pharmaceutical Sciences | May 2021